Visualizing Cryoprotectant Permeation and Location Confined in Plant Cells and Tissues
Abstract: Look out your window and you might see a conifer tree covered with snow. Ever wonder how the needles stay alive all winter long as the temperature dips below freezing? Like pine trees, some organisms possess molecules that naturally enable them to withstand cold. Scientists have copied nature to develop methodology to cryopreserve biological materials, storing them at low temperatures for future use. The first step in any cryopreservation protocol involves dosing the sample with a cocktail of cryopreservation agents, molecules that help them stay viable.