Cryogenic Ion Spectroscopy of the Green Fluorescent Protein Chromophore in Vacuo

Author
Abstract
We present the spectrum of the S1 ← S0 transition of an anionic model for the chromophore of the green fluorescent protein in vacuo at cryogenic temperatures, showing previously unresolved vibrational features, and resolving the band origin at 20 930 cm–1 (477.8 nm) with unprecedented accuracy. The vibrational spectrum establishes that the molecule is in the Z isomer at low temperature. At increased temperature, the S1 ← S0 band shifts to the red, which we tentatively attribute to emergent population of the E isomer.
Year of Publication
2019
Journal
The Journal of Physical Chemistry Letters
Volume
10
Number of Pages
7817-7822
Date Published
2019-11
ISSN Number
1948-7185
URL
https://pubs.acs.org/doi/full/10.1021/acs.jpclett.9b02916
DOI
10.1021/acs.jpclett.9b02916
JILA PI
Associated Institutes
Journal Article