Single-Molecule Kinetic and Thermodynamic Studies of Cosolute-Influenced Nucleic Acid Conformational Transitions

Author
Abstract
<p>Over the last 40 years the number of biochemical functionalities attributed to nucleic acids\&nbsp;<span style="font-size: 13px; line-height: 1.6em;">has increased tremendously. This diverse array of chemical functionality is intimately coupled to\&nbsp;</span><span style="font-size: 13px; line-height: 1.6em;">the spatial arrangement of atoms associated with these molecules. The three-dimensional\&nbsp;</span><span style="font-size: 13px; line-height: 1.6em;">structures and functions of nucleic acids are known to be dependent on the concentration and\&nbsp;</span><span style="font-size: 13px; line-height: 1.6em;">identity of solutes in solution. These nucleic acid cosolutes can be as simple and universal as\&nbsp;</span><span style="font-size: 13px; line-height: 1.6em;">atomic metal cations that favorably interact with the negatively charged phosphate backbone of\&nbsp;</span><span style="font-size: 13px; line-height: 1.6em;">nucleic acids and resulting in stabilization of electronegatively dense conformations.\&nbsp;</span><span style="font-size: 13px; line-height: 1.6em;">Alternatively, they may be complex organic molecules that are able to promote conformational\&nbsp;</span><span style="font-size: 13px; line-height: 1.6em;">transitions in certain RNA sequences responsible for regulating gene expression. Understanding\&nbsp;</span><span style="font-size: 13px; line-height: 1.6em;">the biophysical principles responsible for these cosolutes-influenced conformational transitions\&nbsp;</span><span style="font-size: 13px; line-height: 1.6em;">represents the primary objective of this work.</span></p>
Year of Publication
2014
Degree
Ph.D.
Number of Pages
285
Date Published
2014
University
University of Colorado Boulder
City
Boulder, CO
Advisors - JILA Fellows
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