Single-Molecule Kinetic and Thermodynamic Studies of Cosolute-Influenced Nucleic Acid Conformational Transitions
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Abstract |
<p>Over the last 40 years the number of biochemical functionalities attributed to nucleic acids\ <span style="font-size: 13px; line-height: 1.6em;">has increased tremendously. This diverse array of chemical functionality is intimately coupled to\ </span><span style="font-size: 13px; line-height: 1.6em;">the spatial arrangement of atoms associated with these molecules. The three-dimensional\ </span><span style="font-size: 13px; line-height: 1.6em;">structures and functions of nucleic acids are known to be dependent on the concentration and\ </span><span style="font-size: 13px; line-height: 1.6em;">identity of solutes in solution. These nucleic acid cosolutes can be as simple and universal as\ </span><span style="font-size: 13px; line-height: 1.6em;">atomic metal cations that favorably interact with the negatively charged phosphate backbone of\ </span><span style="font-size: 13px; line-height: 1.6em;">nucleic acids and resulting in stabilization of electronegatively dense conformations.\ </span><span style="font-size: 13px; line-height: 1.6em;">Alternatively, they may be complex organic molecules that are able to promote conformational\ </span><span style="font-size: 13px; line-height: 1.6em;">transitions in certain RNA sequences responsible for regulating gene expression. Understanding\ </span><span style="font-size: 13px; line-height: 1.6em;">the biophysical principles responsible for these cosolutes-influenced conformational transitions\ </span><span style="font-size: 13px; line-height: 1.6em;">represents the primary objective of this work.</span></p>
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Year of Publication |
2014
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Degree |
Ph.D.
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Number of Pages |
285
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Date Published |
2014
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University |
University of Colorado Boulder
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City |
Boulder, CO
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JILA PI Advisors | |
Holmstrom_Thesis_2014-v2.pdf41.1 MB
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Publication Status |