Single Molecule Biophysics: Microscopy, Kinetics and Thermodynamics

Single-Molecule FRET Kinetics of the Mn2+ Riboswitch: Evidence for Allosteric Mg2+ Control of “Induced-Fit” vs “Conformational Selection” Folding Pathways
H. -L. Sung and Nesbitt, D. J., The Journal Of Physical Chemistry B 123, 2005-2015 (2019).
Novel Heat-Promoted Folding Dynamics of the yybP-ykoY Manganese Riboswitch: Kinetic and Thermodynamic Studies at the Single-Molecule Level
H. -L. Sung and Nesbitt, D. J., The Journal Of Physical Chemistry B 123, 5412-5422 (2019).
DNA Hairpin Hybridization under Extreme Pressures: A Single-Molecule FRET Study
H. -L. Sung and Nesbitt, D. J., The Journal Of Physical Chemistry B 124, 110-120 (2020).
Single-Molecule Kinetic Studies of DNA Hybridization Under Extreme Pressures
H. -L. Sung and Nesbitt, D. J., Physical Chemistry Chemical Physics 22, 23491-23501 (2020).
Chirality-Dependent Amino Acid Modulation of RNA Folding
D. A. Nicholson, Sengupta, A., and Nesbitt, D. J., The Journal Of Physical Chemistry B 124, 11561–11572 (2020).
Smaller molecules crowd better: Crowder size dependence revealed by single-molecule FRET studies and depletion force modeling analysis
H. -L. Sung, Sengupta, A., and Nesbitt, D. J., The Journal Of Chemical Physics 154, 155101 (2021).
Pushing Camera-Based Single-Molecule Kinetic Measurements to the Frame Acquisition Limit with Stroboscopic smFRET
D. A. Nicholson and Nesbitt, D. J., The Journal Of Physical Chemistry B (2021).
Sequential folding of the nickel/cobalt riboswitch is facilitated by a conformational intermediate: Insights from single-molecule kinetics and thermodynamics
H. -L. Sung and Nesbitt, D. J., The Journal Of Physical Chemistry B 124, 7348-7360, (2020).
Correction: High pressure single-molecule FRET studies of the lysine riboswitch: cationic and osmolytic effects on pressure induced denaturation
H. -L. Sung and Nesbitt, D. J., Phys. Chem. Chem. Phys. 22, 17008-17009 (2020).
Measuring Excess Heat Capacities of Deoxyribonucleic Acid (DNA) Folding at the Single-Molecule Level
D. A. Nicholson, Jia, B., and Nesbitt, D. J., The Journal Of Physical Chemistry B 125, 9719 (2021).
The Effects of Molecular Crowders on a Single Molecule Nucleic Acid Folding: Temperature Dependent Studies Reveal True Crowding vs. Enthalpic Interactions
D. J. Nesbitt, The Journal Of Physical Chemistry B 125, 13147–13157 (2021).
Lysine-Dependent Entropy Effects in the B. subtilis Lysine Riboswitch: Insights from Single-Molecule Thermodynamic Studies
A. Menendez and Nesbitt, D. J., The Journal Of Physical Chemistry B 126, 69-79 (2022).
Synergism in the Molecular Crowding of Ligand-Induced Riboswitch Folding: Kinetic/Thermodynamic Insights from Single-Molecule Spectroscopy
H. -L. Sung and Nesbitt, D. J., The Journal Of Physical Chemistry B 126, 6419 (2022).
Ligand-Dependent Volumetric Characterization of Manganese Riboswitch Folding: A High-Pressure Single-Molecule Kinetic Study
H. -L. Sung and Nesbitt, D. J., Journal Of Physical Chemistry B 126, 9781 (2022).
Ionic Cooperativity between Lysine and Potassium in the Lysine Riboswitch: Single-Molecule Kinetic and Thermodynamic Studies
A. Menendez and Nesbitt, D. J., The Journal Of Physical Chemistry B 127, 2430 (2023).
Kinetic and Thermodynamic Control of G-Quadruplex Polymorphism by Na<sup>+</sup> and K<sup>+</sup> Cations
D. A. Nicholson and Nesbitt, D. J., The Journal Of Physical Chemistry B 127, 6842–6855 (2023).
Thermodynamic compensation in extreme thermal environments: single molecule dependent kinetic studies of B. subtilis and T.maritima lysine riboswitches
D. J. Nesbitt and Menendez, A., Biophysical Journal (2024).