Optical Tweezers: Light and Life, Studied One Molecule at a Time
Abstract: Prominent among biophysical techniques is the optical trap, for which Arthur Ashkin (Bell Labs) received a Nobel Prize in 2018. Among the successes of optical traps have been direct measurements of the steps taken by biological motor proteins, such as kinesin, and by nucleic-acid enzymes, such as RNA polymerase. Optical traps facilitate studies of replication, transcription, and translation at the single-molecule level. They’ve been especially useful in mapping the free-energy landscapes of folding by small, structured RNAs.