Single Molecule Biophysics: Microscopy, Kinetics and Thermodynamics | JILA

Kinetic and Thermodynamic Origins of Osmolyte-Influenced Nucleic Acid Folding

E. D. Holmstrom, Dupuis, N. F., and Nesbitt, D. J., The Journal Of Physical Chemistry B 119, 3687-3696 (2015).

Single-Molecule FRET Reveals Three Conformations for the TLS Domain of Brome Mosaic Virus Genome

M. Vieweger, Holmstrom, E. D., and Nesbitt, D. J., Biophysical Journal 109, 2625-2636 (2015).

Synthesis and applications of RNAs with position-selective labelling and mosaic composition

Y. Liu, Holmstrom, E. D., Zhang, J., Yu, P., Wang, J., Dyba, M. A., Chen, D., Ying, J., Lockett, S., Nesbitt, D. J., Ferré-D’Amaré, A. R., Sousa, R., Stagno, J. R., and Wang, Y. -X., Nature 522, 368-372 (2015).

Amino Acid Specific Effects on RNA Tertiary Interactions: Single-Molecule Kinetic and Thermodynamic Studies

A. Sengupta, Sung, H. -L., and Nesbitt, D. J., The Journal Of Physical Chemistry B 120, 10615-10627 (2016).

Biophysical Insights from Temperature-Dependent Single-Molecule Förster Resonance Energy Transfer

E. D. Holmstrom and Nesbitt, D. J., Annual Review Of Physical Chemistry 67, 441-465 (2016).

Mechanistic Insights into Cofactor-Dependent Coupling of RNA Folding and mRNA Transcription/Translation by a Cobalamin Riboswitch

J. T. Polaski, Holmstrom, E. D., Nesbitt, D. J., and Batey, R. T., Cell Reports 15, 1100-1110 (2016).

Amino Acid Stabilization of Nucleic Acid Secondary Structure: Kinetic Insights from Single-Molecule Studies

D. A. Nicholson, Sengupta, A., Sung, H. -L., and Nesbitt, D. J., The Journal Of Physical Chemistry B 122, 9869-9876 (2018).

Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA

Y. Liu, Holmstrom, E. D., Yu, P., Tan, K., Zuo, X., Nesbitt, D. J., Sousa, R., Stagno, J. R., and Wang, Y. -X., Nature Protocols (2018).

Tests of Kramers' Theory at the Single-Molecule Level: Evidence for Folding of an Isolated RNA Tertiary Interaction at the Viscous Speed Limit

N. F. Dupuis, Holmstrom, E. D., and Nesbitt, D. J., The Journal Of Physical Chemistry B 122, 8796-8804 (2018).

Single-Molecule FRET Kinetics of the Mn2+ Riboswitch: Evidence for Allosteric Mg2+ Control of “Induced-Fit” vs “Conformational Selection” Folding Pathways

H. -L. Sung and Nesbitt, D. J., The Journal Of Physical Chemistry B 123, 2005-2015 (2019).

Novel Heat-Promoted Folding Dynamics of the yybP-ykoY Manganese Riboswitch: Kinetic and Thermodynamic Studies at the Single-Molecule Level

H. -L. Sung and Nesbitt, D. J., The Journal Of Physical Chemistry B 123, 5412-5422 (2019).