Single Molecule Biophysics: Microscopy, Kinetics and Thermodynamics

Recent Papers in these Areas:

N. Marmé, H. Lee, A. Friedrich, C.-W. Park, D. J. Nesbitt and J.-P. Knemeyer,  “Suppressing nonspecific adsorption of proteins on the single-molecular level,” Proc. SPIE 6862, (2008).

J. L. Fiore, J. H. Hodak, O. Piestert, C. D. Downey and D. J. Nesbitt, “Monovalent and Divalent Promoted GAAA-Tetraloop–Receptor Tertiary Interactions from Freely Diffusing Single-Molecule Studies,” Biophys. J. 95, 3892-3905 (2008).

J. L. Fiore, B. Kraemer, F. Koberling, R. Erdmann, and D. J. Nesbitt, “Enthalpy-driven RNA folding: Single-molecule thermodynamics of tetraloop–receptor tertiary interaction,” Biochemistry 48, 2550-2558 (2009).

E. D. Holmstrom and D. J. Nesbitt, “IR overtone laser temproal control of temperature in picoliter H2O samples: “Nanobathtubs” for single molecule microscopy,” J. Phys. Chem. Lett. 1, 2264-2268 (2010).

J. L. Fiore and D. J. Nesbitt, “An entropic origin of Mg+2–facilitated RNA folding,” Proc. Nat. Acad. Sci. 109, 2902-2907 (2012).

E. D. Holmstrom, J. L. Fiore and D. J. Nesbitt, “Thermodynamic Origins of Monovalent-Facilitated RNA Folding,” Biochemistry 51, 3732-3743 (2012).

E. D. Holmstrom, L. R. Fiegland, J. H. Hodak, and D. J. Nesbitt, “The Role of Counterion Valence and Size in GAAA Tetraloop–Receptor Docking/Undocking Kinetics”, J. Mol. Biol. dx.doi.org/10.1016/j.jmb.2012.07.006 (2012).

L. R. Fiegland, A. D. Garst, R. T. Batey, and D. J. Nesbitt, “Single-Molecule Studies of the Lysine Riboswitch Reveal Effector Dependent Conformational Dynamics of the Aptamer Domain,” Biochem. (submitted).

J. L. Fiore and D. J . Nesbitt, “An RNA folding motif: GNRA tetraloop-receptor interactions,” Quart. Rev. Biophys. (submitted).