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Overcoming Diffraction Limitations in Optical Imaging

Event Details

Event Dates: 

Monday, February 19, 2018 - 4:00pm

Seminar Location: 

  • JILA 10th Floor - Foothills Room

Speaker Name(s): 

Dr. Rafael Piestun
Seminar Type/Subject

Scientific Seminar Type: 

  • Other

Seminar Type Other: 

STROBE Seminar Series

Event Details & Abstract: 

Optical computational imaging seeks enhanced performance and new functionality by the joint design
of illumination, optics, detectors, and reconstruction algorithms. In this talk we discuss how this
approach helps overcome the diffraction limit in fluorescence microscopy. Abbe’s resolution limit has
been overcome after more than 130 years enabling unprecedented opportunities for optical
imaging at the nanoscale. Fluorescence imaging using photoactivatable or photoswitchable
molecules within computational optical systems offers single molecule sensitivity within a
wide field of view from far field measurements. The advent of three-dimensional point
spread function engineering associated with optimal reconstruction algorithms
provides a unique approach to further increase resolution in three dimensions.
Compressive imaging techniques further enable resolution of dense emitters
and enable acceleration of the super-resolution data collection.
Hosted by CU Boulder and broadcast across STROBE nodes.
Everyone is welcome to attend!